DeShields, a Rule 5 draft choice, is hitting .235/.278/.382 with one RBI this spring. Peguero is hitting .350/.435/.500 over 40 at-bats in Cactus League.
Arcia is batting .295 (13 for 44) with one triple, one home run, three doubles and seven RBI in 14 spring games.
It will be the first time Marisnick has cracked the Opening Day roster. The 24-year-old hit .306/.395/.500 this spring and is a candidate to play regularly at center field.
"It's going to feel like Christmas," Marisnick said.
Villar, 23, batted .209 during his second season in the majors in 2014. This spring, he slashed .256/.356/.436 over 39 at-bats.
"He's made some of the most spectacular plays of the spring, both in the outfield and in the infield," general manager Jeff Luhnow said of Villar. "It's a great story."
Harris, 30, will join Houston's bullpen this season after posting a 4.34 ERA last season with the Diamondbacks. He registered a 2.45 ERA, a 1.18 WHIP and 5:2 K:BB ratio over 11 innings this spring.
According to MLB.com, the Astros may try to trade Presley if they are unable to keep him. Presley, 29, slashed .212/.316/.333 with three RBI over 33 spring at-bats.
Singleton, 23, made his debut in 2014 and hit .168 over 310 at-bats in his first season in the majors. He hit .213 this spring.
"There are definitely things I need to improve on," Singleton said. "This move was necessary."
Chapman, 27, registered a 4.64 ERA in his second season in the majors last year. This spring, he posted a 2.16 ERA, a 1.44 WHIP and 10:3 K:BB ratio over 8 1/3 innings.
"I just need to just polish up a little bit," Chapman said.
Crisp was playing in an intrasquad game Tuesday after missing time with the elbow injury, and he appears likely to be shut down again to give the elbow time to heal. Crisp is a candidate to open the season on the disabled list.
Gallo, who was the team's first-round draft pick in 2012, hit .250 in 15 games this spring with two home runs and six RBI. Alfaro hit .313 with four RBI in 16 plate appearances while Mazara hit .286 in seven at-bats.